Detection of extended-spectrum ß-lactamases in members of the family Enterobacteriaceae: comparison of the Combination Double Disc Test Method and the Etest ESBL
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چکیده
Purpose: To evaluate the performance of the Combination Double Disc Method in the identification of ESBL-producing Enterobacteriaceae isolates. Materials and Methods: A total of 44 clinically relevant Enterobacteriaceae isolates were examined. The ESBL classification furnished by Combination Double Disc Test Method was concordant with that of the comparison method (Etest ESBL and molecular identification of beta-lactamase genes) for 18 (41%) of the 44 isolates evaluated. Results: ESBL production was correctly detected in 41% ESBL-producing organisms with at least one of the combination disks (sensitivity, 80%). Conclusion: In our study, Combination Double Disc Test Method appears to be a rapid and reliable tool for routine identification of ESBL-producing isolates of Enterobacteriaceae.
منابع مشابه
Frequency of extended spectrum beta lactamase producer P. aeruginosa strains isolated from burned patients of Motahari hospital, Teharan, Iran
Pseudomonas aeruginosa is an opportunistic pathogen which is naturally resistant to a large range of antibiotics like lots of Beta–Lactams (penicillins, cephalosporins and carbapenems) and may cause additional resistance after unsuccessful treatment. The understanding of beta-lactamase identification and detection in these bacteria is very valuable. In recent years a number of variety of new be...
متن کاملبررسی مقاومت دارویی ناشی از بتالاکتاماز وسیع الطیف (Extended Spectrum ß Lactamases=ESBLs) در اشریشیا کلی با مقاومت چندگانه دارویی در بیماران بستری
Background & Aim: clinical cases especially from hospitalized patients. Recently multiple drug resistant isolated from clinical cases. Resistances were seen against drugs belonging to different antibiotic families. In thissurvey, drug resistance in clinical isolates was studied with special reference to extended spectrum betalactamases.Escherichia coli is one of the most important and prevalent...
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Background & Aims: The production of extended-spectrum-β-lactamases (ESBLs) is the main mechanism of resistance to β-lactam antibiotics. The outbreak of isolates simultaneously possessing several resistance mechanisms to β-lactam antibiotics caused a decrease in sensitivity of the confirmatory tests for ESBL. The aim of this study was the evaluation of the β-lactamase disk test...
متن کاملEvaluation of the β-Lactamase Disk Test Method in the Detection of Extended-Spectrum-β- Lactamases in Clinical Isolates of Multidrug-Resistant Pseudomonas aeruginosa
Background & Aims: The production of extended-spectrum-β-lactamases (ESBLs) is the main mechanism of resistance to β-lactam antibiotics. The outbreak of isolates simultaneously possessing several resistance mechanisms to β-lactam antibiotics caused a decrease in sensitivity of the confirmatory tests for ESBL. The aim of this study was the evaluation of the β-lactamase disk test method in the de...
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